Quality Control of Purified Proteins to Improve Research Data Reproducibility: Improving the Time-Efficiency and Quality of Your Results
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The scientific community aims to enhance transparency and reliability in published research, with a specific focus on the quality of biological reagents, notably recombinant proteins. Concerns have been raised regarding irreproducible results, prompting discussions on common quality issues associated with recombinant proteins and their impact on downstream applications. In response, professionals in protein purification and characterization have collaboratively developed guidelines for improved quality control.
These guidelines include minimal information requirements for protein identity, production parameters, and long-term stability, as well as a minimal set of quality tests for purity, homogeneity, and identity. Additional recommendations cover DNA contamination, “spectral and thermal denaturation signatures”, homogeneity, “competent fraction”, storage conditions, batch-to-batch reproducibility, and other factors based on intended applications.
Evaluation of these guidelines over a one-year period suggests that their implementation can enhance experimental reliability and optimize protein quality. Investing in protein QC benefits all stakeholders in life sciences, including researchers, editors, and funding agencies, by improving data accuracy and minimizing resource wastage
Key learning topics:
The latest guidelines for Quality control of Protein purification and characterisation
How these guidelines can enhance reliability and improve protein quality in your labs
How to reduce wastage and improve data accuracy in protein quality control
Who should attend?
Lab Managers and Scientists working in upstream recombinant protein production and QC
Lab Managers and Scientists working in downstream protein purification QC
Presenter: Dr. André Matagne (Full Professor, University of Liège, Belgium)
Dr. Matange is a Full Professor at the University of Liège, Belgium, where he is the chair at the Centre for Protein Engineering within the Faculty of Sciences.
With a background in biochemistry, his research is primarily focused on proteins, particularly exploring their folding, stability, and biological activity. Notably, he has played a pivotal role in the development of Robotein®, an advanced technological platform designed for to high throughput protein expression, purification, formulation, and analysis.
Since 2016, Dr.Matagnehas been honored as an elected titular member of the Royal Academy of Sciences in Belgium.
