Online Pre-column Derivatization and UHPLC Analysis of Total Amino Acids in Animal Feed

This webinar gives you an introduction on fully automated pre-column derivatization of the amino acids and microwave digestion method which were adopted to further speed up and increase the analysis efficiency.

A Shimadzu Nexera LC-40X3 with photodiode array (PDA) and fluorescence (RF) detectors were employed for the analysis. The Nexera LC-40X3 equipped with automated pre-treatment function allows several derivatization reagents to be aspirated sequentially from multiple vials and mixing within the needle and the sample loop. Microwave digestion procedure was employed to carry out both acid and alkaline hydrolysis in less than 30 minutes, which is much faster in comparison to the conventional hydrolysis up to 24 hours. Fluorescent (RF) detection was used for high sensitivity analysis (nM), while UV detection was compared, which shows its applicability for µM level quantitation of amino acids.

Learning Objectives:

• Background of amino acid analysis in feed

• Comparison between manual vs automated pre-column derivatization

• Sample preparation using conventional hydrolysis vs microwave digestion

Presenter: Liw Wan Tung (Senior Application Chemist, Shimadzu (Asia Pacific) Pte Ltd)

Wan Tung, a senior application chemist, has been with Shimadzu for 8 years. She has 11 years of working experience in analytical science, specializing in HPLC. Wan Tung’s recent research work at Shimadzu has mainly focused on fully automated pre-column derivatization of amino acids in a variety of food and biopharmaceutical sample matrices. Prior to joining Shimadzu, Wan Tung started her professional career as a QAQC chemist in Pharmaceutical multinational company. Wan Tung earned her bachelor’s honours degree in chemistry in 2009.