Direct Probe Ionization Mass Spectrometry (DPiMS) for High Throughput Analysis

Evolution of mass spectrometry (MS) hardware, especially ionization method, has drastically changed the landscape of MS applications.

Direct Probe Ionization Mass Spectrometer (DPiMS) has the potential to pioneer new types of applications with the new ionization interface. The technique successfully incorporated the best of both worlds; high ionization efficiency of ESI and simplicity of MALDI. This was achieved by using an ultrafine needle to sample an extremely small volume of sample on its surface and subsequently applying high voltage to the needle to imitate an ESI probe. DPiMS is characterized by the microscopic sampling volume and high sensitivity for the amount sampled at a low ng/mL concentration range. For this, DPiMS can be a robust solution for the routine analysis of high-complexity matrices, such as plasma or food extract, as contamination of MS hardware can be perfectly mitigated. Moreover, direct sampling from solid samples causing minimal destruction might enable new applications such as real-time analysis of live tissues or cells.

Presenter: Udi Jumhawan (Ph.D.) (Senior Chemist, Shimadzu (Asia Pacific))

Udi has PhD degree in biotechnology specialized in metabolomics field from Osaka University, Japan. He has been joining Shimadzu (Asia Pacific) since 2016 as senior chemist (LCMS).