The role of chromatography and monodisperse particles in mass spectrometry-based metabolomics for disease detection

Metabolomics refers to the comprehensive measurement of small molecules in biofluids by either mass spectrometry (MS) or nuclear magnetic resonance (NMR) with the aim of covering multiple KEGG (Kyoto Encyclopedia of Genes and Genomes) pathways, exposome products, and chemical reactions to provide new insights into disease etiologies.

Lipidomics is a subset of metabolomics focused specifically on the analysis of lipid species. MS-based metabolomics and lipidomics generally require the use of liquid chromatography to separate metabolites based on polarity and high-resolution MS to accurately measure the mass-to-charge (m/z). The combination of retention time and m/z accuracy provides a reliable method to identify metabolites, which is critical for making disease marker discoveries.

In this webinar, Dr. Timothy Garrett, associate professor at the University of Florida, will discuss the clinical translation of metabolomics using examples of different diseases including rare diseases, cancer, and infectious diseases. He will explain how critical chromatography is to reproducibility and metabolite identification, and how new monodisperse columns can aid in separation and identification.

Key learning objectives

• Learn the basics of applied metabolomics using UHPLC-HRMS/MS

• Understand how metabolomics can be used for developing new diagnostic tests

• Understand the potential of metabolomics and the current limitations

• Explore the role of chromatography in metabolomics

• Learn how utilizing monodisperse fully porous particles can improve your current metabolomics method

Who should attend?

• LC scientists looking for more efficiency for their metabolomic separations

• Scientists looking to improve their current HPLC/UHPLC separations for metabolomics

• Chromatographers looking to start developing new metabolomics based HPLC/UHPLC based methods

Presenter: Timothy J. Garrett (Associate Professor, University of Florida)

Dr. Timothy Garrett is an Associate Professor and Chief of Experimental Pathology in the Department of Pathology, Immunology and Laboratory Medicine at the College of Medicine at The University of Florida. He received his undergraduate degree from the University of Georgia in Chemistry and his PHD in Chemistry from the University of Florida. He joined the University of Florida in 2006, where he has developed a research program in metabolomics, lipidomics, and small molecule quantitation for clinical and biological research using mass spectrometry approaches. He is also currently Co-Editor-in-Chief for the Journal of Mass Spectrometry and Advances in the Clinical lab.

Presenter: Carrie Haslam (SelectScience)

Dr. Carrie Haslam is an Associate Editor at SelectScience, playing a key role in content production and specializing in Materials Science, Alzheimer’s disease and Clinical Diagnostics. Carrie completed a Ph.D. from The University of Plymouth, where she developed graphene-based biosensors for the early diagnosis of Alzheimer’s disease.