Conquer Method Variability: Evaluate Variables During Method Development
There are many variables to consider during method development, some more obvious than others. Laying the groundwork early with clear goals will help you develop robust and rugged methods that are easier to use, validate, and transfer.
In this presentation, we will look at:
How to assess the effect of varying a parameter
Adjustments you can make to address variability
Chemistry and instrument configuration factors that can affect the robustness and ruggedness of HPLC separations
A rugged, robust method is not only cost effective in the long term, but it also increases the likelihood of having a method that will pass with flying colors when you transfer it to other in-house or contract analytical laboratories.
Presenter: Rita Steed (Application Engineer, Agilent Technologies, Inc.)
Rita Steed began supporting the LC column line for Agilent in 1999. As a Chromatography Specialist (first with Chromatography, Inc., then directly for Agilent), Rita worked on-site with researchers in Pharmaceutical and other industries presenting technical seminars and assisting researchers with troubleshooting and method development. Rita has over 20 years of Chromatography experience in the Biotechnology, Chemical, and Pharmaceutical industries. She has held positions in Research, Sales, and Technical Service. Rita has earned degrees in Microbiology and Life Sciences/Biochemistry. In her current position, she is an inside Application Engineer supporting LC columns.
