Getting Off to a Good Start: The Makings of a Reliable Method

When the robustness of an HPLC method has not been tested, it often results in the inability of others to reproduce it.

If your method will be used by others, it’s important to know it will perform as expected when it’s out of your hands.

In this presentation, we’ll look at several key factors and conditions to assess their potential effect on a method. Things like column choice and lot, mobile phase factors, including pH, and the instrument you’re using all play an important role when you’re developing a method that will stand the test of time. Avoid frustration in the future by paying attention to these key factors in the present.

Presenter: Rita Steed (Application Engineer, Agilent Technologies, Inc.)

Rita Steed began supporting the LC column line for Agilent in 1999. As a Chromatography Specialist (first with Chromatography, Inc., then directly for Agilent), Rita worked on-site with researchers in Pharmaceutical and other industries presenting technical seminars and assisting researchers with troubleshooting and method development. Rita has over 20 years of Chromatography experience in the Biotechnology, Chemical, and Pharmaceutical industries. She has held positions in Research, Sales, and Technical Service. Rita has earned degrees in Microbiology and Life Sciences/Biochemistry. In her current position, she is an inside Application Engineer supporting LC columns.