Characterizing critical quality attributes of outer membrane vesicles for conjugate vaccines
With a size comparable to that of viruses and self-adjuvating properties, outer membrane vesicles (OMVs) represent an excellent alternative to traditional carrier proteins for conjugate vaccines. An essential aspect of developing OMVs as vaccine products is characterization with respect to size and purity. Asymmetric-flow field-flow fractionation coupled with multi-angle light scattering (AF4-MALS) is an invaluable technique for bionanoparticle analysis, with an AF4-MALS method for quantifying OMV critical quality attributes recently being published in Analytical Chemistry.
This webinar will introduce AF4-MALS and OMV, and review the validation program that was established to assess the quality attributes obtained by AF4-MALS according to the principles described in ICH Guidelines Q2 (R1).
Key learning objectives
- Understand the principles and technology of AF4-MALS
- Discover the strategies and parameters for AF4-MALS method development
- Learn how to assess OMV quality attributes by AF4-MALS
- Consider AF4-MALS validation using ICH guidelines
Who should attend?
- Scientists developing analytical methods for bionanoparticles and vaccines
- Vaccine development scientists in need of robust, reliable, methods to characterize size and purity
Certificate of attendance
All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.
Presenter: Robert van der Put (Intravacc B.V.)
Robert van der Put, MSc., is a conjugate vaccine development scientist at Intravacc where he has worked on the development of an OMV-long-peptide vaccine targeting COVID-19 and a peptide conjugate vaccine against ALS. He is involved in the transfer of a Hib conjugate vaccine production process to partners in Asia and has done both in-house and on-site training of international partners for vaccine production, conjugation, purification and quality control testing. Robert is currently studying at the University of Utrecht for a PhD, whilst holding the position of Director of Business Development at Intravacc.
Presenter: Roger Scherrers, Ph.D. (Wyatt Technology)
Dr. Roger Scherrers studied chemistry at the Heinrich-Heine University in Düsseldorf, where he also completed his PhD. Roger has been with Wyatt Technology Europe since 2007 where he is head of the Application Laboratory. Robert specializes in solving analytical challenges for macromolecules and nanoparticles using light scattering in combination with size-based separation methods such as SEC (size-exclusion chromatography) and FFF (field-flow fractionation).
Presenter: Jemima Arnold (Editorial Team, SelectScience)
Jemima studied Cellular and Molecular Medicine at the University of Bristol, UK, and as a member of the Editorial team, she now plays an integral role in shaping the content on SelectScience.
