Thermo Scientific™ µPAC™ Neo HPLC Columns – Pushing Proteomics Boundaries with micro-Pillar Chromatography

Though the increase in sampling depth and proteome coverage can be accomplished by rapid advances in mass spectrometer technology, appropriate selection of nano LC column is critical to provide MS instruments with peptides separation at the highest possible resolution.

Recently introduced Thermo Scientific micro pillar array column (µPAC) offers proteomics researchers with enhanced chromatographic performance, stability, and robustness in their nanoLC-MS workflows.

In this webinar, we will present the next generation Thermo Scientific µPAC Neo columns, specifically designed to further push the nanoLC separations to achieve deeper proteomic coverage with shorter runtime.

What will I take away from the event?

• Learn about micro-Pillar Array Column (µPAC) technology.
• See how to best employ µPAC Neo columns in your nanoLC-MS workflow.
• Overcome challenges like retention time stability and robustness in your nanoLC chromatography.

Presenter: Julia Kraegenbring (Product Specialist LC-MS, Thermo Fisher Scientific)

Julia Kraegenbring received her PhD from the Westfaelische Wilhelms University in Muenster, Germany. Since she started at Thermo Fisher Scientific, Julia has become a product specialist on the Orbitrap Exploris platforms for proteomics applications, currently focused on the development of high throughput data independent workflows for protein identification and quantitation.

Presentation Title: Velocity LFQ Data Independent Acquisition (DIA) Platform

Presenter: Pedro Aragón Fernández (PhD student, Technical University of Denmark)

Pedro Aragón holds a bachelor’s and master’s degree in biochemistry from the Technical University of Munich (TUM). He developed an interest in analytical chemistry for biological applications and continued to pursue a PhD in the Cell Diversity Lab supervised by Dr. Erwin M. Schoof at the Technical University of Denmark (DTU). His current work focuses on the development and implementation of novel preparative and analytical strategies for mass spectrometry-based single-cell proteomics to understand the synergy displayed by phenotypically distinct subpopulations in the hematopoietic system.

Presentation Title: Enhancing limited sample- and single-cell proteomics through tailored Data-Independent Acquisition and micropillar array-based chromatography.

Presenter: Dr. Jeff Op de Beeck (Staff Scientist, Research & Development, Thermo Fisher Scientific)

Dr. Jeff Op de Beeck is currently Staff Scientist at Thermo Fisher Scientific’s R&D facility in Ghent where micro pillar array technology is developed and produced. His work under supervision of Prof. Gert De Smet and Prof. Wim De Malsche formed the basis for the current micro Pillar Array Column, which is branded by Thermo Fisher Scientific as the micro-Pillar Array Column (µPAC™). He holds a master’s degree in Biomedical Sciences from the university of Antwerp and a Ph.D. in Chemical Engineering from the university of Brussels.