Pushing low-flow chromatography to optimize proteomics workflows
Low-flow chromatography has established itself as the main separation technique used in MS-based proteomics. Especially when performing bottom-up or shotgun proteomics of highly complex peptide mixtures, the possibility to perform most efficient separation followed by sensitive MS detection is key. A recent innovation in nanoLC, the Thermo Scientific™ µPAC™ HPLC Column, now offers the opportunity to push chromatographic separations, resulting in higher peak capacities and increased protein identifications.
Utilizing its unique micro-pillar backbone, µPAC HPLC Columns are operated at much reduced back pressures. Consequently, longer columns can be used, which allow scientists to dive deeper into the proteome. Another advantage is the remarkable reproducibility of µPAC HPLC Columns. Generated with a photo-lithographic etching process, tight control over pillar positioning and dimensions allows for separation channels that always consist of the same number of pillars, located at the same well defined and perfectly ordered position, resulting in virtually identical copies.
This Webinar Will Focus On
- Overview of bottom-up and shotgun proteomics
- Advances in low-flow chromatography columns
- The benefits of pillar array technology
Key Learning Objectives
- Understand the advantages of low-flow chromatographyUnderstand what a pillar array column is
- Understand what a pillar array column is
- How to gain more information about your proteomics sample
Who Should Attend
- Academic proteomics researchers
- Low-flow LC-MS operators
- Biopharmaceutical early development researchers
- Lab managers
Presenter: Robert van Ling (Product Marketing Manager Chromatography Consumables, Thermo Fisher Scientific)
Robert van Ling has 20+ years of experience in nanoLC separations for proteomics LC-MS. In previous roles, he has participated in the introduction in a variety of low-flow products, like the Thermo Scientific™ UltiMate™ 3000 Systems and Thermo Scientific™ PepMap™ and EASY-Spray™ Columns. In the last few years, Robert has focused his activities on the development and commercialization of µPAC HPLC columns, and is keen to share his experience.
Presenter: Kristen Craven (Marketing Manager, Analytical Services Group, Thermo FIsher Scientific)
