Illuminating Targeted Metabolomic Approaches: Ways to Make it Work for your Particular Needs
Absolute Quantitation of Fragile Metabolites using Ion Pair Chromatography and Isotope Dilution Mass Spectrometry
This Webinar focuses on using ion pair chromatography with isotope dilution mass spectrometry to quantitate fragile metabolites. To address the challenge of analyzing fragile unstable analytes, Agilent has redesigned the ion funnel on the Agilent 6495C triple quadrupole LC/MS. The concentrations we show range from attomole to nanomole levels in biological matrices covering the wide range of concentrations that are observed in biological samples. The use of 13C incorporated yeast avoids the cost and time-consuming labor of preparing isotopically labeled standards. Using the ratio of the response from the 12C analyte to the ratio of the response of the 13C-labeled analyte, the linear dynamic range of quantitation extends to between 6–8 orders of magnitude.
Presenter: Sheher Banu Mohsin, Ph.D. (Senior Applications Scientist, Agilent Technologies, Inc.)
Sheher Mohsin is a senior applications scientist at Agilent Technologies. She received her Ph. D in physical chemistry from the University of Illinois and an MBA from Rockhurst University. She started her career at the US Environmental Protection Agency working on dioxin analysis with high resolution mass spectrometers. She later joined Bayer and worked in the special analysis lab using mass spectrometry to solve problems in synthesis, impurity determination and submission of final product impurity profile to regulatory agencies. Sheher’s current focus is on lipidomics using GC, LC and SFC separations and mass spectrometry. Sheher collaborates with academic and government researchers working on complex problems to come up with innovative, simplified workflows using the latest tools in separation and mass spectrometry.
A Turnkey, End-to-End Targeted LC/MS Metabolomics and Lipidomics Workflow
LC/MS-based omics workflows continue to evolve and improve due to various technological developments in each area of the workflow. This webinar brings together technological advances in sample preparation and analyte separation from biocrates and technological advances in analyte detection from Agilent to provide a comprehensive, targeted LC/MS omics workflow for the detection of hundreds of lipids and polar metabolites. The sample preparation method and data collection are supported by several data analysis software packages offered by both biocrates and Agilent that can be used to extract compounds of interest, calculate key metabolic ratios, and complete statistical comparisons.
Presenter: Genevieve Van de Bittner, Ph.D. (R&D Researcher, Agilent Research Laboratories, Agilent Technologies, Inc.)
Genevieve joined the Agilent Research Laboratories within Agilent Technologies in 2016 with a goal of improving technologies for metabolomic and metabolism measurements. Toward this goal, Genevieve has developed new sample preparation methods for LC/MS metabolomic and lipidomic analyses. These efforts have been informed by Genevieve’s training in chemical biology, specifically in the development of novel chemical probes to study biological systems. In her graduate work in Professor Chris Chang’s Lab at UC Berkeley, Genevieve created bioluminescent and fluorescent sensors for the in vivo measurement of reactive oxygen species and metal ions. This training led to a postdoctoral fellowship in Professor Jacob Hooker’s Lab at Harvard Medical School, during which Genevieve developed new radiotracers for imaging specific neuronal populations.
Jump Starting Your Targeted Metabolomics Workflow: New Methods for Automated Sample Prep, HILIC Separation, and Comprehensive MRM Database
Targeted metabolomics methods can be deployed for profiling or quantitating known metabolites in a biological sample. Developing the methodology can be troublesome for new users or busy labs that are focused on biological insights and not LC/MS method development. Discussed here is an established methodology for solid phase extraction sample prep, reproducible HILIC chromatography, and a custom MRM database for polar metabolites that includes retention times. Data from plasma and cell matrices will be showcased as well and downstream analysis for biological interpretation. This talk will focus on a targeted metabolomic workflows for answering your research questions with ease.
Presenter: Karen Yannell (LC/MS Application Engineer, Agilent Technologies, Inc.)
Karen E. Yannell is a LC/MS Application Engineer at Agilent Technologies. She is an expert in small molecule high throughput analysis by triple quadrupole (TQ) and quadrupole time-of-flight (QTOF) mass spectrometry for applied markets. Her recent work has focused on developing routine Q-TOF workflows for both expert and non-expert users alike. Previously, Karen worked on forensic, metabolomics, and biomarker discovery applications with ambient ionization sources. She received her PhD in Analytical Chemistry from Purdue University where studied under the advisement of Prof. R. Graham Cooks. Outside of the laboratory, she enjoys cooking unique dishes and hiking along the beach with her dog.
