Whisper is here

Whisper is the latest Evosep One innovation that allow plug-and-play, split-free 100 nl/min performance. The new methods make use of Evosep’s unique low pressure gradient formation and very accurate flow control on the same standard system without technical modifications.

The gradient is built and stored in the loop in the low pressure part of the system and then pushed to the analytical column using the isocratic high pressure pump, allowing for accurate gradients at very low flow. The Whisper methods are designed for ultra high sensitivity applications, where sample amounts are very limited. The methods are freely available through a software upgrade for standard Evosep One hardware, while optimized columns and emitters are required.

Learn more about the advantages of the pioneering Whisper™ flow technology in this webinar.

Proteomics at the single cell level with Evosep Whisper and the timsTOF SCP

Recent developments in trapped ion mobility spectrometry (TIMS) coupled to fast and sensitive mass spectrometry established in the timsTOF SCP enables proteomics at the single cell level. The Evosep Whisper 100 40 samples per day (SPD) method was applied to picogram to low nanogram peptide loads of HeLa cell lysate digests on Evotips and was acquired in Data Independent Acquisition with Parallel Accumulation and SErial Fragmentation (diaPASEF). This combination demonstrates fast and robust chromatographic separation, high protein and peptide identification reproducible with accurate quantification while allowing for a comprehensive proteome coverage.

Presenter: Christoph Krisp (Scientist at Bruker)