Simplify Your Nutritional Measurements - Simultaneous Determination of Fat-Soluble Vitamins in Foods by Agilent LC-MS/MS

Accurate quantitative measurements for naturally occurring fat-soluble vitamins in foods are required in nutritional chemistry and food industry. A simple and robust method based on direct saponification followed by Agilent Chem Elut Supported Liquid Extraction (SLE) was developed for the simultaneous determination of fat-soluble vitamins on Agilent 1290 Infinity II LC coupled to an Agilent 6470 triple quadrupole LC/MS system in positive ionization mode. The fat-soluble vitamins include Vitamin A (retinol), Vitamin D (Cholecalciferol (D3) and Ergocalciferol (D2)) and Vitamin E (Alpha-Tocopherol). This method was validated and successfully applied for determination of fat-soluble vitamins in a various complex food samples including infant formula, egg, tuna, mushroom, and pet food.

Presenter: Hui Zhao (Pre-Sales Application Engineer, Agilent Technologies, Inc.)

Hui has 13 years of industry experience developing and validating analytical methods for food/feed nutrition, food safety, dietary supplements and botanicals testing, using a variety of analytical techniques including LC-TQ, LC-QTOF, LC-DAD and GC-MS and a breadth of sample preparation methodologies. She has worked as a Research Scientist at Monsanto, EPL-Bioanalytical Services, Tate & Lyle, Inc. and Lead Staff Scientist at Covance Food Solutions. She holds a Master of Science degree in Analytical Chemistry from Lanzhou University in China and a Ph.D. in Analytical Chemistry from the University of Missouri.

Prior to becoming a Pre-Sales AE, Hui worked as an LCMS application scientist in Agilent’s global market development group where her primary focus was food and environmental market development.