Size exclusion native mass spectrometry for the characterization of monoclonal antibodies
Size exclusion chromatography (SEC) is a well-established method for routine aggregate analysis of monoclonal antibodies (mAbs) and other related biotherapeutics. However, SEC can be combined with high-resolution mass spectrometry (HRMS) to obtain even more detailed characterization data for mAbs. So-called native SEC-MS techniques determine mAb higher order structure, relative abundance of glycoforms, and fragmentation.
In this SelectScience webinar, Brian Rivera will discuss how non-denaturing SEC Native MS can be leveraged to streamline mAb characterization. Column selection, including pore volume and column dimensions, are also covered to ensure monomer and aggregate. Optimization of volatile mobile phase composition is shown for improvements in both MS sensitivity and chromatographic performance. Finally, methods for digestion of mAbs using site-specific proteases to obtain further insight on post-translational modifications will be described.
During this webinar, you will learn:
- How SEC Native MS might be used to provide orthogonal data to other analytical methods
- The critical role of column selection in SEC Native MS
- How to improve MS method sensitivity for volatile mobile phases used in SEC Native MS
Presenter: Brian Rivera (Phenomenex)
Brian Rivera is a Senior Product Manager at Phenomenex, with over 10 years in HPLC analysis of large molecules. Before joining Phenomenex, his previous industry experience includes positions at Chiron Corporation (now Novartis), ProZyme (now Agilent), and Avid Bioservices, performing protein purification as well as analytical methods development and in-process analytical support. His primary focus was on protein characterization by size exclusion, glycan mapping, and intact reversed phase HPLC methods. He has a Bachelor’s Degree from the University of California, Davis.
Presenter: Cameron Smith (SelectScience)
Cameron studied Biomedical Science at the University of the West of England, UK, specializing in medical microbiology and oncology during his final year. As a member of the Editorial team, he now plays an integral role in shaping the content on SelectScience.
