Measuring AAV Quality Attributes with Light Scattering

Adeno-associated virus (AAV) is a common delivery vehicle in gene therapy. Robust, rapid and automated characterization methods are imperative for commercialization of AAV-based products. However, standard methods are labor- and time-intensive, requiring costly reagents. In this presentation, we will discuss a method to measure three critical quality attributes (CQAs) of AAVs: 1) total number of viral capsid particles; 2) relative capsid content (e.g., fraction of full capsids); and 3) percentage of monomer or aggregates.

Who should attend and why?

• Scientists and managers, working in the gene and cell therapy field, in need of robust, reliable, simple, and fast methods to characterize and quantify viral vectors.
• CMC regulatory specialists seeking to identify validatible analytical tools that are orthogonal and complementary to current CQA quantification methods.
• Managers of academic labs and core facilities developing viruses and non-viral nanoparticles, that need straightforward biophysical methods to quantify gene vectors.

Learning objectives:

• How light scattering combines with SEC and other detectors to provide comprehensive biophysical analysis of AAVs
• How SEC-MALS can be positioned as a platform technology in AAV development and production

Presenter: John Champagne (Senior Application Scientist, Wyatt Technology)

Senior Applications Scientist and Northeast Regional Manager for Wyatt Technology, currently runs the applications lab for Wyatt Technology in the Boston area and provides both sample analysis and customer support services. He graduated with a M.Sc. (2000) and Ph.D. (2009) from the Biochemistry Department at the University of New Hampshire, under the advisory of Professor Thomas Laue. Since joining Wyatt in 2002, John has provided a range of analytical support with expertise in the use of multi-angle static and dynamic light scattering, field flow fractionation, analytical ultracentrifugation, circular dichroism spectroscopy, UV-Vis spectroscopy, fluorescence spectroscopy and calorimetry.