Too Polar for Reverse Phase: What Do You Do?
Analyzing polar molecules presents a unique challenge for chromatographers. These molecules span the gamut of varying markets: from Polar pesticides in food to Non-steroidal anti-inflammatory drugs to highly polar sugars and amino acids. Conventional HPLC stationary phases like C18 often do not provide adequate retention and separation of such highly polar compounds.
Consequently, chromatographers are forced to step outside of their comfort zone and start exploring polar chemistries. Both Reverse phase polar columns as well as hydrophilic stationary phases are utilized for solving this problem.
This webinar will not focus on specific applications, but we will try to present general guidelines on how to choose the right separation mode (HILIC or RP). Working down a checklist, we will discuss aspects that have to be taken into consideration when developing new polar molecule separations.
In this webinar, we will discuss how to:
- Find the best column to retain and separate polar analytes, focusing on HILIC and polar RP
- Choose between RP and HILIC based on analyte solubility and sample solvent
- How the right separation mode can ensure reliable detection of your sample
Presenter: Sunil B Lakhmapure (Technical Support Specialist Consumables, Agilent Technologies Debesh)
Holds 18 year plus experience in the analytical field with major exposure to LC/GC with Single quad , Triple Quad and High resolution (QToF) mass spectroscopy.
Started career in Pharma R&D as a scientist with hands on experience on high end instruments like LCMS, XRD & NMR .
Developed & published application notes using Chromatographic techniques on real world samples/issues at various levels.
