Getting the Best Out of Solid-Core Technology for Small and Large Molecule Analysis

The growth of solid-core particle technology in liquid chromatography has seen an increase in the applicability of these types of particles to a wider range of workflow solutions. Initial offerings were primarily targeted towards small molecule separations. More recently, however, wide pore solid core particles have been released onto the market, designed specifically for the separation of large biomolecules. Additionally, there has also been an increase in the diversity of stationary phases available to the separation scientist.

The use of different pore sizes and pore volumes and the impact this has on separation performance, as well as the column loading capacity will be discussed. This will be done for a range of different molecular mass molecules and the impact of pore size and pore volume will be demonstrated, with a particular focus on the separations of biomolecules.

Example separations will include intact and partially digested mAbs, as well as a full tryptic digest. The protocols for the digestion will be discussed and the impact of different column chemistries will be investigated to demonstrate the importance of assessing a range of column chemistries during method development, even for large biomolecule separations.

The final part of the presentation will look at some more unusual aspects that can occur in the field of chromatography, such as the influence of flow rate on retention factor. It might be considered that the flow rate does not have an impact on the relative retention time of an analyte, but this is not always the case. This will be discussed in the context of method transfer, along with factors such as equilibration time with specific reference to solid core materials.

Key Learning Objectives

• Learn the importance of pore size for biomolecular separations
• Learn the fundamental benefits of solid-core technology
• Review examples of large and small molecule separations for solid-core method development

Who Should Attend

• Researchers involved in method development from all industries

Presenter: Matt James, Ph.D. (Senior Research Scientist, Avantor)

Moderator: Ann Thayer (Contributing Editor, C&EN Media Group)