What It Takes to Achieve a Good Chromatography – Selecting the Right Column Chemistries and Mobile Phase

Separation Science, in collaboration with Waters, offers a webinar comprising two presentations looking first at column attributes and how to gain a better understanding of how best to select a column for a specific application and second, the importance of mobile phase selection in the separation process.

Column Attributes: What’s Really Important for Chromatography?

Advancements in LC column development, specifically with base particle and chemistry, have brought forth a wide selection of analytical columns for scientists to choose from. Though many vendors provide columns that often use the same chemistry, 'C18' for instance, different C18 columns from different vendors often perform quite differently in terms of retention and selectivity. In this presentation we’ll look at the key attributes of both the base particle and bonded chemistry, and how they contribute to the separation. By understanding these column attributes, we can gain a better understanding on how best to select a column for a specific application.

Presenter: Jonathan Turner (Principal Product Marketing Manager, Analytical Columns, Waters Corporation)

Jonathan is the Product Marketing Manager for analytical UPLC, UHPLC, and HPLC columns at Waters Corporation. In this role, he uses his expertise in chromatographic media to help design, develop, and commercialize new modern stationary phases.

Back to Basics: Mobile Phase Selection in HPLC and UHPLC

Much focus is given to the stationary phase in liquid chromatography; however, the mobile phase also has an important role in the separation process. This presentation will discuss the impact mobile phase can have on selectivity in reversed phase chromatography, and the importance of selecting the correct mobile phase components, whether that be methanol, acetonitrile or even tetrahydrofuran. The discussion will move onto the use of isocratic and gradient elution systems, and the benefits and disadvantages of each of these approaches will be highlighted. An approach to method development using a scouting gradient will also be presented.

Presenter: Tony Edge (Site Director - Production & R&D, Hichrom)

Tony Edge is an R&D Manager at Avantor Sciences, heading a team of specialist scientist in developing next generation stationary phases for HPLC. He has worked in both manufacturing and also industry.

By attending this webinar you will...

• gain an understanding of different column particle technologies, their specific attributes and influences on chromatographic separation
• learn about the effect of mobile phase on selectivity and its use in method development