Advancement of Analytical Technology for Oligonucleotide Analysis Using High-Resolution Accurate-Mass Mass Spectrometry
The advancement of oligonucleotide therapeutics and mRNA vaccine drives the need to develop robust analytical approaches for confident identification and in-depth characterization of these novel modalities.
We will demonstrate the capability of Thermo Scientific™ Vanquish Horizon™ UHPLC system and DNAPac™ RP column for robust and reproducible separation of oligonucleotides under the denaturing and non-denaturing conditions.
We will highlight the power of Thermo Scientific™ Orbitrap Exploris™ mass spectrometers for accurate mass measurement of oligonucleotides as large as 100mer.
Additionally, we will describe a data dependent MS/MS (ddMS2) method for high confidence identification, mapping, and relative quantitation of oligonucleotides and their impurities within a single experiment.
The characterization of degradation products and unexpected high molecular weight impurities of a synthetic oligonucleotide will also be examined. Data analysis using Thermo Scientific™ BioPharma Finder™ Software will also be explored, highlighting newly designed and novel tools to support the analysis and visualization of MSMS data for both modified and unmodified oligonucleotide workflows.
Key learning objectives:
- Discover new LC-MS methods for oligonucleotide and RNA analysis.
- Understand how a data dependent MS/MS (ddMS2) method can be applied to oligonucleotide identification, mapping, relative quantitation and impurity analysis.
- Find out how BioPharma Finder™ Software can support your oligonucleotide analysis.
Who should attend;
- Biopharmaceutical Research Scientists
- Analytical Scientists from Biopharma companies working on or interested in oligonucleotide based therapies
- PhD students in any field of biotherapeutic drug development
- Scientists looking to advance knowledge of oligonucleotide analysis
Presenter: Dr. Kevin Guo (Senior Application Scientist, Thermo Fisher Scientific)
Dr. Kevin Kun Guo is a Senior Applications Scientist at Thermo Fisher Scientific with 18 years of experience running LC-MS systems, specializing in pharmaceutical and biopharmaceutical applications support. Before joining Thermo Fisher he was a Mass Spectrometry Scientist at Immunogen and completed his Ph.D. at the University of Alberta.
Presenter: Keeley Murphy (Software Product Manager, Thermo Fisher Scientific)
Keeley Murphy joined Thermo Fisher Scientific in 2008 and is currently working in the role of Software Product Manager, with a focus on the development of software workflows to support the LCMS analysis of oligonucleotides and mRNA. With 12 years of experience within Thermo Fisher Scientific, Keeley has contributed to numerous product launches and introductions for both hardware and software technologies designed to support biopharmaceutical development.
