Improving the sensitivity of LC-MS methods for characterization and quantitation of synthetic oligonucleotides
The primary method for quantitation and characterization of synthetic oligonucleotides is liquid chromatography-mass spectrometry (LC-MS). Whether for characterizing process and product-related impurities or bioanalytical pharmacokinetic studies, improving method sensitivity is of critical importance.
He will also demonstrate how to minimize adduct formation and enhance ionization efficiency, which can in turn increase MS sensitivity for improved characterization and quantitation data.
Key learning objectives:
- Learn the fundamentals of electrospray ionization of oligonucleotides, and how different method parameters can affect performance
- Understand the role of mobile phase and column chemistry in LC-MS methods for oligonucleotides
- Determine experimental parameters to ensure high-sensitivity, robust LC-MS methods for synthetic oligonucleotides
Who should attend?
- Analytical method developers looking to improve separation and method robustness for oligonucleotides
- Bioanalytical scientists needing to improve method sensitivity
- Anyone new to oligonucleotide characterization by LC-MS
Presenter: Brian Rivera (Senior Product Manager- Biologics, Phenomenex)
Brian Rivera is a Senior Product Manager at Phenomenex, with over 10 years in HPLC analysis of large molecules. Before joining Phenomenex, his previous industry experience includes positions at Chiron Corporation (now Novartis), ProZyme (now Agilent), and Avid Bioservices, performing protein purification as well as analytical methods development and in-process analytical support. His primary focus was on protein characterization by size exclusion, glycan mapping, and intact reversed phase HPLC methods.
He has a Bachelor’s Degree from the University of California, Davis.
Moderator: Finn Price (Editorial Team, SelectScience)
Fionnbhar Price is a member of the SelectScience Editorial Team, who plays a core role in sourcing and publishing content for the site, with a particular focus on the field of applied chemistry. He has an MChem in Chemistry from Cardiff University, UK, and undertook a year in research characterizing bacterial protein.
